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Replication of Oryctes Baculovirus in Cell Culture: Viral Morphogenesis, Infectivity and Protein Synthesis

Entomology Division, Department of Scientific and Industrial Research, Private Bag, Auckland, New Zealand

The sequence of events in the replication of Oryctes baculovirus in DSIR-HA-1179 cells began with the uptake of virus particles by pinocytosis at the plasma membrane. Seven h after infection, enveloped virus particles were observed inside a cleared area in the nucleus. Virus was released from cells by budding through the plasma membrane where a second unit membrane was acquired. The infectivity of virus particles with a single envelope purified from within cells was not significantly different from that of virus which had acquired the second envelope by budding. Twenty-seven structural virus proteins were identified by gradient polyacrylamide gel electrophoresis. The synthesis of eight of the major structural proteins was detected by pulse labelling infected cells with L-[³⁵S]methionine. No late protein synthesis, as observed in subgroup A baculoviruses, was detected in this subgroup C baculovirus.

Keywords: Oryctes rhinoceros, baculovirus, morphogenesis, protein synthesis

Received 20 July 1984; accepted 23 October 1984.