HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Van Beek, N. A. M. Articles by Dijkstra, J. Search for Related Content PubMed Articles by Van Beek, N. A. M. Articles by Dijkstra, J. Agricola Articles by Van Beek, N. A. M. Articles by Dijkstra, J.

Polyethylene Glycol-mediated Infection of Cowpea Protoplasts with Sonchus Yellow Net Virus

Nikolai A. M. Van Beek

Anja C. G. Derksen

Department of Virology, Agricultural University, Binnenhaven 11, 6709 PD Wageningen, The Netherlands

The conditions favouring the infection of cowpea mesophyll protoplasts by Sonchus yellow net virus (SYNV) were determined. When 3 x 10⁶ protoplasts were inoculated with 60 µg SYNV in 40% polyethylene glycol, 3 mM-CaCl₂ at room temperature, over 90% of the surviving protoplasts became infected. Infectivity tests showed that the virus could be detected 12 h after inoculation.

Keywords: SYNV, protoplast infection, polyethylene glycol

Present address: Boyce Thompson Institute for Plant Research at Cornell University, Tower Road, Ithaca, N.Y. 14853, U.S.A.

Received 9 July 1984; accepted 7 November 1984.