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Cauliflower Mosaic Virus DNA Persists as Supercoiled Forms in Cultured Turnip Cells

¹ Dipartimento di Biologia Cellulare, Universitá degli Studi di Camerino, via F. Camerini 2, Camerino 62032, Italy
and² Department of Virus Research, John Innes Institute, Colney Lane, Norwich NR4 7UH, U.K.

Explants taken from turnip leaves infected systemically with cauliflower mosaic virus (CaMV) proliferate callus tissue when cultured on an appropriate medium. CaMV DNA, detected by spot hybridization, was found to persist in these cultured cells for at least 45 days. Analysis of unencapsidated virus DNA by blot hybridization after agarose gel electrophoresis showed that the truncated DNA forms characteristic of infected leaf tissue were virtually absent from callus cells. These cells contained predominantly genome-length supercoiled CaMV DNA together with discrete subgenomic supercoiled forms.

Keywords: CaMV, callus culture, supercoiled DNA

Received 19 October 1984; accepted 27 November 1984.