J Gen Virol Tips for Better Browsing
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 66 (1985), 621-626; DOI 10.1099/0022-1317-66-3-621
© 1985 Society for General Microbiology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chernos, V. I.
Right arrow Articles by Senkevich, T. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chernos, V. I.
Right arrow Articles by Senkevich, T. G.
Agricola
Right arrow Articles by Chernos, V. I.
Right arrow Articles by Senkevich, T. G.

Recombinants between Vaccinia and Ectromelia Viruses Bearing the Specific Pathogenicity Markers of Both Parents

Victor I. Chernos, Tamara P. Antonova and Tatiana G. Senkevich

Research Institute for Viral Preparations, 15, 1st Dubrovskaya St., Moscow 109088, U.S.S.R.

Nineteen recombinants between vaccinia virus (VV) DNA- temperature-sensitive mutants and ectromelia virus (EMV) were characterized with respect to their biological properties and genome structure. Four of these recombinants acquired the pathogenicity for mice characteristic of EMV, while the pathogenicity for rabbits characteristic of VV was not only preserved, but even enhanced. Most unexpectedly, these recombinants with ‘double pathogenicity’, as well as four other recombinants, acquired a stable genetic trait which was not typical of the parental viruses, i.e. the ability to form haemorrhagic lesions on the chorioallantoic membrane of chick embryos. Approximate mapping of the genomes of these recombinants with restriction endonucleases showed that their DNA contained mostly VV sequences with a single detected insert of EMV DNA. In the ‘double pathogenicity’ recombinants, this insert was located in the central part of the genome, and its minimal size was about 16 Mdal.

Keywords: vaccinia virus, ectromelia virus, interspecies recombinants, pathogenicity

Received 8 August 1984; accepted 8 November 1984.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1985 by the Society for General Microbiology.