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J Gen Virol 66 (1985), 693-700; DOI 10.1099/0022-1317-66-4-693
© 1985 Society for General Microbiology
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Stimulation of Fibroblast Interferon Production by a 22K Protein from Human Leukocytes

J. Van Damme1, G. Opdenakker1, A. Billiau1, P. De Somer1, L. De Wit2, P. Poupart2 and J. Content2

1 Rega Institute for Medical Research, University of Leuven, Minderbroedersstraat 10, B-3000 Leuven
and2 Institut Pasteur du Brabant, 642, rue Engeland, B-1180 Brussels, Belgium

We have studied the appearance of human interferon-beta (HuIFN-beta) as well as its mRNA in cells treated with a protein, 22K factor, isolated from the culture supernatant of mitogen-stimulated human peripheral blood leukocytes. By itself 22K was found to be unable to induce production of significant amounts of HuIFN-beta protein. However, when aided by treatment with cycloheximide or cycloheximide and actinomycin D (superinduction), 22K caused increases in production ranging from 3- to 20-fold, depending on the cells (diploid or MG-63 osteosarcoma) and the induction schedule. Cells treated with 22K alone produced small amounts of HuIFN-beta mRNA, which was only detectable with a highly sensitive method. In combination with cycloheximide, 22K induced levels of mRNA detectable with less sensitive methods as well. These experiments provide further support for the concept that the antiviral activity of 22K is mediated by its ability to stimulate transcription of the HuIFN-beta gene in cells.

Keywords: interferon (HuIFN-beta), inducer, leukocytes

Received 23 July 1984; accepted 13 December 1984.




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Copyright © 1985 by the Society for General Microbiology.