HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Binns, M. M. Articles by Brown, T. D. K. Search for Related Content PubMed PubMed Citation Articles by Binns, M. M. Articles by Brown, T. D. K. Agricola Articles by Binns, M. M. Articles by Brown, T. D. K.

Cloning and Sequencing of the Gene Encoding the Spike Protein of the Coronavirus IBV

Houghton Poultry Research Station, Houghton, Huntingdon, Cambs. PE17 2DA
and¹ Department of Biochemistry, University of Leeds, Leeds LS2 9JT, U.K.

RNA sequences encoding the surface projection (spike) of the coronavirus infectious bronchitis virus, strain Beaudette, have been cloned into pBR322 using cDNA primed with a specific oligonucleotide. A 5.3 kilobase viral insert in the clone pMB179 has been identified. The region of this clone coding for the spike gene has been sequenced by the chain termination method, and we present here the first report of DNA sequence data for a coronavirus spike protein, the protein which forms the characteristic ‘corona’ after which the group is named. The amino acid sequence of the primary translation product, deduced from the DNA sequence, predicts a polypeptide of 1162 amino acids with a molecular weight of 127006. This has many interesting features which confirm and extend our knowledge of this recently characterized membrane glycoprotein. The polypeptide is subsequently cleaved to S1 and S2, and partial amino acid analysis of the amino-terminus of the S1 polypeptide has been employed to locate the position of this terminus of S1 within the large open reading frame. The amino acid analysis also reveals the presence of an 18 amino acid putative signal sequence on the primary translation product which is not present on the mature S1 polypeptide.

Keywords: coronavirus IBV, spike protein, DNA sequence, mRNA E

Received 25 October 1984; accepted 19 December 1984.

This article has been cited by other articles:

				C. A. M. de Haan, B. J. Haijema, P. Schellen, P. W. Schreur, E. te Lintelo, H. Vennema, and P. J. M. Rottier Cleavage of Group 1 Coronavirus Spike Proteins: How Furin Cleavage Is Traded Off against Heparan Sulfate Binding upon Cell Culture Adaptation J. Virol., June 15, 2008; 82(12): 6078 - 6083. [Abstract] [Full Text] [PDF]

				S. Jitrapakdee, S. Unajak, N. Sittidilokratna, R. A. J. Hodgson, J. A. Cowley, P. J. Walker, S. Panyim, and V. Boonsaeng Identification and analysis of gp116 and gp64 structural glycoproteins of yellow head nidovirus of Penaeus monodon shrimp J. Gen. Virol., April 1, 2003; 84(4): 863 - 873. [Abstract] [Full Text] [PDF]

				K. Stirrups, K. Shaw, S. Evans, K. Dalton, R. Casais, D. Cavanagh, and P. Britton Expression of reporter genes from the defective RNA CD-61 of the coronavirus infectious bronchitis virus J. Gen. Virol., July 1, 2000; 81(7): 1687 - 1698. [Abstract] [Full Text]

				K. Stirrups, K. Shaw, S. Evans, K. Dalton, D. Cavanagh, and P. Britton Leader switching occurs during the rescue of defective RNAs by heterologous strains of the coronavirus infectious bronchitis virus J. Gen. Virol., March 1, 2000; 81(3): 791 - 801. [Abstract] [Full Text]