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A Reappraisal of the Effect of Actinomycin D and Cordycepin on the Multiplication of Cowpea Mosaic Virus in Cowpea Protoplasts

John G. Shaw

John Innes Institute, Colney Lane, Norwich NR4 7UH, U.K.

Actinomycin D (AD) administered to cowpea mosaic virus-infected cowpea protoplasts immediately after inoculation inhibited virus multiplication, whereas late in the incubation period neither virus multiplication nor host DNA transcription were affected. The data obtained suggested that neither virus uncoating nor encapsidation were inhibited by AD. The inhibition of virus multiplication was manifested as a decrease in the level of progeny viral nucleoproteins and (+) and (-) viral RNAs. Cordycepin strongly inhibited coat protein production and (+) and (-) viral RNA synthesis throughout the incubation period.

Keywords: CPMV, protoplasts, actinomycin D, cordycepin

Present address: Department of Plant Pathology, S-305 Agricultural Science Building-North, University of Kentucky, Lexington, Kentucky 40546-0091, U.S.A.

Received 16 October 1984; accepted 19 December 1984.