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J Gen Virol 66 (1985), 839-844; DOI 10.1099/0022-1317-66-4-839
© 1985 Society for General Microbiology
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Comparison of RNA from Healthy and Scrapie-infected Hamster Brain

Thomas L. German1, Blair C. McMillan2, Brian E. Castle2, Craig Dees2, William F. Wade2 and Richard F. Marsh2

1 Department of Plant Pathology, 1630 Linden Drive
and2 Department of Veterinary Science, University of Wisconsin-Madison, Madison, Wisconsin 53706, U.S.A.

Density gradient fractions prepared from healthy or scrapie-infected hamster brain tissue enriched in plasma membrane vesicles were treated with nucleases prior to phenol extraction and ethanol precipitation. The recovered nucleic acids were 3' end-labelled and run on one-dimensional polyacrylamide gels. Autoradiography revealed the presence of low molecular weight RNAs (4S) in both healthy and scrapie samples. Two-dimensional fingerprint analysis indicated that the RNAs isolated from scrapie-infected hamsters contained oligonucleotides that were not present in RNAs isolated from healthy hamsters.

Keywords: scrapie, RNA, oligonucleotides

Received 2 August 1984; accepted 15 November 1984.




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