J Gen Virol
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J Gen Virol 66 (1985), 851-859; DOI 10.1099/0022-1317-66-4-851
© 1985 Society for General Microbiology
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Characterization of Proteins in Membrane Vesicles from Scrapie-infected Hamster Brain

C. Dees, T. L. German, W. F. Wade and R. F. Marsh

Department of Veterinary Science, 1655 Linden Drive, University of Wisconsin-Madison, Madison, Wisconsin 53706, U.S.A.

Previous studies have shown that the scrapie agent is highly membrane-associated. We examined the protein composition of gradient fractions enriched for large membrane vesicles prepared from scrapie-infected and uninfected hamster brain using various methods to extract membrane proteins. We also examined proteins in detergent-extracted membrane vesicles fractionated on CsCl gradients. No qualitative differences in protein composition were seen comparing scrapie-infected and uninfected samples by one-dimensional gel electrophoresis. Extraction of proteins from membrane vesicles by phenol, pyridine, perchloric acid or lithium diiodosalicy-late also failed to reveal any unique proteins in scrapie-infected hamster brain. Attempts to solubilize hydrophobic proteins (proteolipids) from CsCl gradient fractions into organic solvents were unsuccessful. These findings indicate that any hydrophobic protein associated with the scrapie agent is not a proteolipid, and that the ability of solvents to reduce scrapie infectivity is not a result of extraction of a proteolipid.

Keywords: scrapie, membrane vesicles, hydrophobic protein

Received 18 September 1984; accepted 31 December 1984.




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