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Human Interferons Alpha and Beta Have More Potent Priming Activities than Interferon Gamma

Miklós Tóth

Institute of Microbiology, University Medical School, H-6720 Szeged, Dóm tér 10, Hungary

The priming activities of human IFN-, IFN- and IFN- were compared on the same antiviral basis using human buffy coat leukocytes stimulated with Sendai virus or concanavalin A (Con A) to produce IFN- or IFN-, respectively. Pretreatment of leukocytes with any type of IFN enhanced their IFN- and IFN- production, but IFN- and IFN- had more potent priming activities than IFN-. IFN- and IFN- did not potentiate the priming activity of each other in either the IFN-- or the IFN--producing system. Pretreatment of leukocytes with relatively high doses of IFN- or IFN- (1000 to 3000 IU/ml) resulted in a 40- to 50-fold increase in the IFN- production of Con A-stimulated leukocytes. This observation will be of use in producing IFN- with a high titre.

Keywords: interferon (HuIFN), priming, leukocytes

Present address: Institute of Biochemistry, Biological Research Centre, Hungarian Academy of Sciences, P.O. Box 521, H-6721 Szeged, Hungary.

Received 13 August 1984; accepted 29 November 1984.