HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Hsu, Y. H. Articles by Brakke, M. K. Search for Related Content PubMed Articles by Hsu, Y. H. Articles by Brakke, M. K. Agricola Articles by Hsu, Y. H. Articles by Brakke, M. K.

Sequence Relationships among Soil-borne Wheat Mosaic Virus RNA Species and Terminal Structures of RNA II

Agricultural Research Service, U.S. Department of Agriculture and Department of Plant Pathology, Nebraska Agricultural Experiment Station, University of Nebraska, Lincoln, Nebraska 68583, U.S.A.

Gel-separated RNAs of the wild-type (WT), Lab 1 and Lab 2 isolates of soil-borne wheat mosaic virus (SBWMV) were transferred to nitrocellulose filters and hybridized with specific cDNA. RNA I, but not RNA II, from all three SBWMV isolates hybridized to cDNA copied from SBWMV WT RNA I. RNA II from all three SBWMV isolates hybridized to cDNA copied from SBWMV WT RNA II, confirming the hypothesis that the Lab 1 and Lab 2 isolates arose by deletion of part of RNA II of WT virions. RNA II from SBWMV WT (0.5L RNA) or from SBWMV Lab 1 (0.35L RNA) did not bind to oligo(dT)-cellulose in high-salt buffer, or stimulate cDNA synthesis when primed with oligo(dT), suggesting that it has no 3'-poly(A) or oligo(A) sequences. No genome-linked protein was detected at the 5' ends of either RNA II by iodination, nor did the cap analogue 7-methylguanosine phosphate have an effect on their translation in vitro, indicating no cap structure at their 5' ends. RNA II was 5' end-labelled with [³²P]ATP and T4 polynucleotide kinase after dephosphorylation with bacterial alkaline phosphatase, but all four bases were labelled, indicating heterogeneity at the 5' end.

Keywords: SBWMV, RNA species, sequence relationships

Received 5 September 1984; accepted 17 December 1984.