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J Gen Virol 66 (1985), 925-930; DOI 10.1099/0022-1317-66-4-925
© 1985 Society for General Microbiology
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Double-stranded, Replicative Form RNA Molecules of Cowpea Mosaic Virus Are Not Infectious

M. Shanks, G. P. Lomonossoff and D. Evans{dagger}

John Innes Institute, Colney Lane, Norwich NR4 7UH, U.K.

Virus-specific double-stranded replicative form (RF) RNA was isolated from cowpeas infected with cowpea mosaic virus. The RF was assayed for infectivity in a local lesion host and a systemic host. In neither host was undenatured RF infectious, although infectivity was restored upon denaturation.

Keywords: CPMV, replicative form RNA

{dagger} Present address: Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, U.K.

Received 11 October 1984; accepted 11 January 1985.




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Copyright © 1985 by the Society for General Microbiology.