HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Stanley, J. Articles by Curson, S. J. Search for Related Content PubMed Articles by Stanley, J. Articles by Curson, S. J. Agricola Articles by Stanley, J. Articles by Curson, S. J.

Pseudorecombinants between Cloned DNAs of Two Isolates of Cassava Latent Virus

Department of Virus Research, John Innes Institute, Colney Lane, Norwich NR4 7UH, U.K.

Infective clones of the Nigerian isolate of cassava latent virus (CLV) have been obtained. The apparent molecular weight of the capsid protein of this isolate is slightly higher than that produced in plants infected with cloned DNAs of the Kenyan isolate of CLV. Pseudorecombinant experiments using heterologous combinations of cloned DNAs have confirmed that the physical properties of the capsid protein are encoded on DNA 1 and at least some determinants of symptom induction are also located on this DNA. Comparison between the nucleotide sequences of the open reading frames encoding the two capsid proteins shows several nucleotide differences which affect the amino acid composition but which do not significantly alter the potential molecular weight of the product. In vitro translation of poly(A)⁺ RNAs shows that the differences in electrophoretic mobility are not due to differences in host-directed post-translational processing.

Keywords: CLV, geminivirus, pseudorecombinants, infectivity of DNA clones

Received 5 November 1984; accepted 4 January 1985.

This article has been cited by other articles:

				S. Chakraborty, R. Vanitharani, B. Chattopadhyay, and C. M. Fauquet Supervirulent pseudorecombination and asymmetric synergism between genomic components of two distinct species of begomovirus associated with severe tomato leaf curl disease in India J. Gen. Virol., March 1, 2008; 89(3): 818 - 828. [Abstract] [Full Text] [PDF]

				J. A. Willment, D. P. Martin, K. E. Palmer, W. H. Schnippenkoetter, D. N. Shepherd, and E. P. Rybicki Identification of long intergenic region sequences involved in maize streak virus replication J. Gen. Virol., June 1, 2007; 88(6): 1831 - 1841. [Abstract] [Full Text] [PDF]