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A Dot Immunobinding Assay for the Detection of Tobacco Mosaic Virus in Infected Tissues

Laboratory of Applied Microbiology, Department of Applied Physiology, National Institute of Agrobiological Resources
and¹ Division of Microbiology, Department of Environmental Biology, National Institute of Agro-Environmental Sciences, Tsukuba Science City, Yatabe, Ibaraki 305, Japan

A dot immunobinding assay (DIBA) was modified for the rapid detection of plant viruses in infected tissues by avoiding non-specific reactions and minimizing the amounts of antibodies used. With this method, less than 1 ng of tobacco mosaic virus could be detected in several milligrams of infected tobacco leaves. This simple, rapid and sensitive assay should prove to be a useful and practical diagnostic technique for plant virus diseases.

Keywords: DIBA, TMV, dot ELISA, ELISA

Received 21 August 1984; accepted 31 December 1984.