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J Gen Virol 66 (1985), 945-955; DOI 10.1099/0022-1317-66-5-945
© 1985 Society for General Microbiology
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Recognition of mos-related Proteins with an Antiserum to a Peptide of the v-mos Gene Product

G. E. Gallick1, J. T. Sparrow2, B. Singh3, S. A. Maxwell3, L. H. Stanker4 and R. B. Arlinghaus3

1 UTSCC M.D. Anderson Hospital, Department of Tumor Biology, Houston, Texas 77030
2 Baylor College of Medicine, Department of Medicine, Houston, Texas 77030
3 Scripps Clinic and Research Foundation, Department of Molecular Biology, La Jolla, California 92037
and4 Lawrence Livermore National Laboratory, Livermore, California 94550, U.S.A.

A mos-specific antiserum was generated by injection of rabbits with a peptide predicted from the sequence of the v-mos gene of Moloney murine sarcoma virus (MuSV) strain 124. The peptide is composed of amino acids 37–55 (cyclized at the cysteine residues) conjugated to keyhole limpet haemocyanin. This serum [anti-mos(37–55c)] specifically recognized p37mos in MuSV-124 acutely infected NIH-3T3 cells, P85gag-mos in 6m2 cells, an NRK clone infected with the temperature-sensitive mutant (ts110) of Moloney MuSV, and P100gag-mos in 54-5A4 cells, an NRK clone infected with a spontaneous revertant of ts110. An additional protein of Mr 55000 from uninfected cells was recognized by this serum. Reactivity of the serum toward the v-mos-containing proteins and the 55K protein was completely inhibited by prior incubation with free peptide. The 55K protein was not recognized by antisera made from synthetic peptides prepared from the C-terminal eight or 12 amino acids of v-mos.

Keywords: v-mos gene, Mo-MuSV, protein recognition, peptide antibody

Received 6 June 1984; accepted 21 December 1984.




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Copyright © 1985 by the Society for General Microbiology.