HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Curran, J. A. Articles by Rima, B. K. Search for Related Content PubMed PubMed Citation Articles by Curran, J. A. Articles by Rima, B. K. Agricola Articles by Curran, J. A. Articles by Rima, B. K.

cDNA Cloning of the Nucleocapsid and Nucleocapsid-associated Protein Genes of Mumps Virus

Department of Biochemistry, The Queen's University of Belfast, Belfast BT9 7BL, U.K.

cDNA clones of the mumps virus N and P messenger RNAs were isolated from an infected cell cDNA library. The N and P clones selected the two predominant polyadenylated RNAs found in mumps virus-infected cells with mol. wt. of 0.69 x 10⁶ and 0.51 x 10⁶, respectively. In addition, clones of the P gene hybridized to and selected mRNAs of higher mol. wt. probably representing polycistronic transcripts of the mumps genome. Hybrid-select translation experiments confirmed the specificity of the clones as representing the nucleocapsid (N) and nucleocapsid-associated protein (P) genes.

Keywords: mumps virus, cDNA cloning, nucleocapsid

Received 16 October 1984; accepted 14 January 1985.