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Influence of C-terminal Modifications of øX174 Lysis Gene E on its Lysis-inducing Properties

Udo Bläsi

Werner Lubitz

Fachbereich Biologie, Universität Kaiserslautern, Postfach 3049, D-6750 Kaiserslautern, Federal Republic of Germany

The øX174 gene E product (gpE) causes lysis of Escherichia coli by inducing the host autolytic system. Experiments were carried out to ascertain which part of the 91 amino acid polypeptide carries the functional site for this process. For this purpose fusion genes were created comprising the first 51 codons of gene E and unrelated sequences coding for 102 or 33 amino acids respectively. The chimeric protein of 153 amino acids consisting of the N-terminal part of gpE and a fragment of -galactosidase, was neither able to lyse E. coli nor to restore -galactosidase activity by -complementation. Expression of the 84 amino acid polypeptide, however, was able to induce lysis of E. coli. It is therefore concluded that the functional lysis-inducing site of gpE is located within the cloned N-terminal part of gene E. In the shorter chimeric protein the sequence following the functional site was tolerated or necessary for stabilization, but in the longer chimeric protein, the C-terminal sequence disturbed the lysis-inducing conformation.

Keywords: phage øX174, lysis function, chimeric proteins

Present address: Institute of Genetics and Microbiology, University of Munich, Maria-Ward-Strasse la, D-8000 München 19, F.R.G.

Received 11 December 1984; accepted 18 February 1985.

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