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Isolation of Haemorrhagic Fever with Renal Syndrome Virus from Leukocytes of Rats and Virus Replication in Cultures of Rat and Human Macrophages

¹ Department of Pediatrics, Fujita-gakuen Hoken-eisei University, School of Medicine, Toyoake, Aichi 470-11
² Kannonji Institute, Research Foundation for Microbial Diseases, Osaka University, Kannonji, Kagawa 768
³ Department of Tuberculosis Research II
⁴ Quarters for Experimentally Infected Animals
and⁵ Department of Virology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565, Japan

Newborn rats were inoculated intraperitoneally with haemorrhagic fever with renal syndrome (HFRS)-related virus (B-1 strain), and virus isolation from their various organs was attempted between 1 and 25 weeks after inoculation. Virus could be isolated repeatedly from lung, brain, spleen and kidney and also from peripheral blood. When virus isolation was carried out on fractionated peripheral blood cells, virus was associated mainly with the macrophage fraction and to a lesser extent with granulocytes. Virus replicated well in peritoneal exudate cells of normal rats and it grew in the adherent mononuclear cells from normal human peripheral blood. These data suggest that macrophages, permissive for HFRS-related virus replication, might contribute to the spread of viral infection in vivo.

Keywords: HFRS, Hantaan virus, rats

Received 20 November 1984; accepted 8 February 1985.

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