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Nucleotide Sequence of the Respiratory Syncytial Virus Phosphoprotein Gene

Department of Microbiology, University of Southampton Medical School, Southampton General Hospital, Southampton SO9 4XY, U.K.

A cDNA library was prepared from poly(A)⁺ RNA extracted from respiratory syncytial (RS) virus-infected HEp-2 cells. A recombinant plasmid, pRSP68, encoding the RS virus phosphoprotein gene was identified by translation in vitro of hybrid-selected mRNA. The cloned cDNA insert of 1 kb was sequenced and a polypeptide of 241 amino acids with a molecular weight of 27166 was deduced from the sequence. The protein was relatively rich in polar amino acids and devoid of both cysteine and tryptophan. A second short open reading frame with a coding potential of 65 amino acids was identified and overlapped the 3' terminus of the phosphoprotein gene by 34 bases.

Keywords: RS virus, phosphoprotein, sequence

Received 11 February 1985; accepted 27 March 1985.

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