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J Gen Virol 66 (1985), 1687-1695; DOI 10.1099/0022-1317-66-8-1687
© 1985 Society for General Microbiology
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Antigenic Determinants of Influenza Virus Haemagglutinin. X. A Comparison of the Physical and Antigenic Properties of Monomeric and Trimeric Forms

Ann Nestorowicz, Graeme Laver1 and David C. Jackson

Department of Microbiology, University of Melbourne, Parkville, Victoria 3052
and1 Unit for Influenza Research, John Curtin School for Medical Research, Australian National University, Canberra, A.C.T. 2601, Australia

Haemagglutinin prepared from influenza virus A/Memphis/1/71 by bromelain digestion was centrifuged through continuous sucrose gradients buffered at pH 7.4 or pH 4.9. From these gradients were isolated two forms of the protein which displayed different equilibrium sedimentation properties. One species behaved as a molecule with a mol. wt. of 190000, the other with a mol. wt. of 70000. These results are consistent with the separation of trimeric and monomeric haemagglutinin. A comparison of their antigenic properties, using monoclonal antibodies raised against intact virus, showed that major antigenic differences occur between the two forms of haemagglutinin. None of the monoclonal antibodies reacted with haemagglutinin denatured by reduction and alkylation.

Keywords: influenza A virus, haemagglutinin, antigenicity

Received 13 March 1985; accepted 7 May 1985.


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