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J Gen Virol 66 (1985), 1733-1740; DOI 10.1099/0022-1317-66-8-1733
© 1985 Society for General Microbiology
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Definition of Three Serotypes of Hantaviruses by a Double Sandwich ELISA with Biotin-Avidin Amplification System

D. Goldgaber, C. J. Gibbs, Jr, D. C. Gajdusek and A. Svedmyr1

Laboratory of Central Nervous System Studies, National Institute of Neurological and Communicative Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20205, U.S.A.
and1 Central Microbiological Laboratory of Stockholm County, P.O. Box 177, S-101 22 Stockholm, Sweden

A total of seven different isolates of Hantaviruses from five mammalian species and six geographical areas were examined by a double sandwich ELISA with biotin-avidin amplification system. Three serotypes were recognized by comparing antigen titres to homologous and heterologous antibody and blocking antibody titres to homologous and heterologous antigens. Serotype 1 included four strains: Hantaan virus isolated from Apodemus agrarius in South Korea, Hupei-I isolated from acute-phase serum from a patient with epidemic haemorrhagic fever (EHF) in China, SR-11 isolated from a laboratory rat associated with an EHF outbreak in Sapporo, Japan, and Tchoupitoulas isolated from a wharf rat (Rattus norvegicus) trapped in New Orleans, La., U.S.A. Serotype 2 included two strains of nephropathia epidemica virus, Hällnäs-I and Puumala, isolated from Clethrionomys glareolus trapped in nephropathia epidemica foci in Sweden and Finland, respectively. Serotype 3 included one strain, Prospect Hill-I, isolated from Microtus pennsylvanicus, trapped in Frederick, Md., U.S.A.

Keywords: Hantaan virus, serotypes, ELISA, biotin-avidin

Received 31 December 1984; accepted 18 April 1985.




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Copyright © 1985 by the Society for General Microbiology.