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Susceptibility to Herpes Simplex Virus Type 1 Infection of Non-permissive Rat XC(HPRT^-) x Permissive Mouse L(TK^-) Hybrid Cells

Unité de Virologie Fondamentale et Appliqueé, INSERM U.51, Groupe de Recherche C.N.R.S. 33, 1, place Professeur Joseph Renaut, 69371 Lyon Cedex 2, France

Somatic cell hybrids between rat XC(HPRT^-) cells, non-permissive for herpes simplex virus type 1 (HSV-1) infection, and permissive mouse L(TK^-) cells were constructed and karyotyped. Infection of these hybrid cells by HSV-1 strains F and MP revealed that they were susceptible to the virus. The amounts of virus produced by the hybrid cells, as well as the cytopathic effect observed, was very similar to that of the parental L(TK^-) cells. Our results suggest that failure of HSV-1 to replicate in XC cells is more likely to be due to the absence of cellular elements required for efficient virus multiplication rather than to the presence of blocking or inhibiting factors.

Keywords: HSV-1, XC cells, permissive hybrid cells

Received 20 December 1984; accepted 8 May 1985.