HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Bush, C. E. Articles by Pritchett, R. F. Search for Related Content PubMed PubMed Citation Articles by Bush, C. E. Articles by Pritchett, R. F. Agricola Articles by Bush, C. E. Articles by Pritchett, R. F.

A Comparison of the Genomes of Bovine Herpesvirus Type 1 and Pseudorabies Virus

Department of Veterinary Microbiology and Immunology, University of California, Davis, California 95616, U.S.A.

The DNA sequence homology between bovine herpesvirus type 1 (BHV-1) and pseudorabies virus (PRV) was examined. Reciprocal cross-hybridization of viral DNA labelled by nick translation to Southern blots of restriction endonuclease-digested DNA detected homologous sequences dispersed throughout the genomes of the two viruses. The DNA-DNA hybrids formed were stable under high-stringency wash conditions. Sequences of a ³²P-labelled PRV DNA fragment probe were found to hybridize only to a specific region of the BHV-1 genome, suggesting that the detected sequence homology was not due to fortuitous hybridization of G + C-rich sequences. As measured by liquid reassociation kinetics the homology between these two viruses was approximately 8%.

Keywords: BHV-1, PRV, blot hybridization, reassociation kinetics

Received 14 December 1984; accepted 12 April 1985.