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Transcription of the Bacillus subtilis Bacteriophage ø3T in vitro

Enda Kenny

Centre for Biotechnology, Imperial College, London SW7 2AZ
and¹ Microbial Technology Laboratory, PHLS Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire SP4 0JG, U.K.

The in vitro transcription pattern of BglII-digested ø3T DNA is described. Eight BglII fragments that hybridized to in vitro transcription products were unequivocally identified. A further hybridizing region corresponding to a BglII triplet was also revealed, giving a total of nine to 11 BglII fragments. These represent 47 to 53% of the ø3T genome. Transcription was shown to initiate within BglII fragments B, G, C, H, I, F and J, implying that all of these contain at least one promoter. The relevance of these data to the construction of a cloning vector based on ø3T is discussed.

Keywords: phage ø3T, promoter, Southern blot, transcription mapping

Present address: Delta Biotechnology Ltd, 137 High Street, Burton-on-Trent DE14 1JZ, U.K.

Received 28 January 1985; accepted 30 May 1985.