J Gen Virol Try Microbiology Online
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 67 (1986), 107-118; DOI 10.1099/0022-1317-67-1-107
© 1986 Society for General Microbiology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tsutsui, Y.
Right arrow Articles by Furukawa, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tsutsui, Y.
Right arrow Articles by Furukawa, T.
Agricola
Right arrow Articles by Tsutsui, Y.
Right arrow Articles by Furukawa, T.

Monoclonal Antibodies to Guinea-pig Cytomegalovirus: An Immunoelectron Microscopic Study

Yoshihiro Tsutsui, Yoshiro Yamazaki, Akiko Kashiwai, Akira Mizutani and Toru Furukawa1

Institute for Developmental Research, Aichi Prefectural Colony, Kasugai, Aichi 480-03
and1 Department of Microbiology, Kanazawa Medical University, Uchinada, Ishikawa 920, Japan

Three groups of monoclonal antibodies which reacted with cells infected by guinea-pig cytomegalovirus (GPCMV) were prepared. The first group of antibodies immunoprecipitated a 50000 mol. wt. (50K) polypeptide of GPCMV-infected cells. This polypeptide was identified as part of the nuclear inclusion by immunofluorescence. This nuclear fluorescence was markedly diminished when the infected cells were incubated in the presence of phosphonoacetic acid. By immunoelectron microscopy the antibodies reacted mainly with filamentous structures (26 to 28 nm in diameter) in nuclear inclusions and occasionally stained nucleocapsids. Neither intracytoplasmic nor extracellular virions reacted with the antibodies. Therefore, the 50K protein with which the monoclonal antibodies reacted was nuclear inclusion-specific and a non-structural protein. The second group of antibodies reacted with a 76K polypeptide of the infected cells which was a matrix protein found in both cytoplasmic inclusions and extracellular dense virions. The third group of antibodies mainly reacted with a virion core protein by immunoelectron microscopy.

Keywords: GPCMV, monoclonal antibodies, immunoelectron microscopy

Received 6 June 1985; accepted 24 September 1985.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1986 by the Society for General Microbiology.