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Studies on the Major Common Precipitating Antigen of Capripoxvirus

Animal Virus Research Institute, Pirbright, Woking, Surrey GU24 0NF, U.K.

The proteins of sheep pox, goat pox, sheep and goat pox and lumpy skin disease (Neethling) viruses were labelled with [³⁵S]methionine. The major structural polypeptides of these viruses co-migrated on polyacrylamide gels, demonstrating the very close biochemical relationship between them. Using the agar gel immunodiffusion (AGID) test with radiolabelled antigen preparations, a major common precipitating antigen was identified. This co-migrated on polyacrylamide gels with one of the major structural polypeptides [mol. wt. 67000 (67K)]. The use of [³⁵S]methionine-labelled antigen preparations considerably improved the sensitivity of the AGID test as a diagnostic test for capripoxvirus antibody detection.

Keywords: capripoxviruses, antigens, AGID

Received 14 June 1985; accepted 24 September 1985.

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