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Herpesviruses Provide Helper Functions for Avian Adeno-associated Parvovirus

Institute of Poultry Diseases, Free University of Berlin, Koserstrasse 21, 1000 Berlin 33, F.R.G.

The avian herpesviruses infectious laryngotracheitis virus (ILTV) and herpesvirus of turkeys (HVT), as well as the mammalian herpesvirus pseudorabies virus (PRV) were able to provide complete helper activity for the production of infectious avian adenoassociated virus (AAAV) in chicken cells. The presence of AAAV in the infected chicken cell reduced the multiplication of HVT. ILTV or PRV, however, were not affected if used as helper viruses. Infectious AAAV was determined by an indirect immunofluorescence assay and infectious herpesvirus by plaque assays.

Keywords: AAAV, herpesviruses, helper viruses

Received 24 June 1985; accepted 30 September 1985.

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