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5S RNA and tRNA-like Molecules Are Associated with Killer Virus dsRNA of Yeast

Department of Microbiology, University of Medicine and Dentistry of New Jersey, Rutgers Medical School, Piscataway, New Jersey 08854, U.S.A.

A population of oligonucleotides co-purified with the dsRNA genomic segments of the killer virus of Saccharomyces cerevisiae during electrophoresis through agarose gels. These smaller RNA molecules must be separated from the viral genome in order to determine the structure of the dsRNA molecules. Sequence analysis of these isolated oligonucleotides showed that the population contained tRNA-like molecules, as well as 5S RNA, which are presumably encoded by the host cell genome.

Keywords: oligonucleotides, tRNA, yeast, killer virus

Received 31 May 1985; accepted 26 September 1985.