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Characterization of Monoclonal Antibodies against Potato Virus X and Comparison of Serotypes with Resistance Groups

MAFF Harpenden Laboratory, Hatching Green, Harpenden, Herts. AL5 2BD
and¹ The Monoclonal Antibody Centre, AFRC Institute of Animal Physiology, Babraham, Cambridge CB2 4AT, U.K.

The production and testing of 10 rat monoclonal antibodies specific for strains of potato virus X (PVX) are described. Monoclonal antibodies (MAbs) with a variety of specificities were selected. At one extreme, MAb MAC 58 reacted with all 33 PVX isolates tested; in contrast, MAb MAC 67 reacted strongly with only two of 33 isolates, one of which was the resistance-breaking PVX-HB against which it was raised. The reactions of the PVX isolates with the MAbs were compared with the classification of PVX strains based on their effects in plants carrying different resistance genes; a preliminary serological classification is suggested. It is possible to distinguish PVX-HB from all other isolates tested using two MAbs (MAC 67 and MAC 59), and these, together with MAC 58, were produced in large quantities in ascitic fluids. Together they provide a valuable set of reagents for use in routine testing.

Keywords: PVX, monoclonal antibodies, serological groups

Received 4 June 1985; accepted 24 September 1985.