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In vitro Translation of the Bipartite Genomic RNA of Pea Early Browning Virus

G. Hughes^1,

¹ Department of Microbiology, University of Birmingham, Birmingham B15 2TT
and² Department of Virus Research, John Innes Institute, Colney Lane, Norwich NR4 7UH, U.K.

Translation of the two genomic RNAs of a British strain (SP5) of pea early browning virus (PEBV-B) in rabbit reticulocyte lysates produced three principal polypeptides of M_r 165 x 10³ (165K), 134K and 23K. The two largest polypeptides were produced by RNA-1 alone, and evidence is presented which suggests that the 165K polypeptide arises from the 134K product by a readthrough event. RNA-2 directed the translation of the 23K polypeptide, which was immunoprecipitated by anti-PEBV-B serum. A polypeptide of M_r 24.3K, produced by translation of the genomic RNAs of the closely related broad bean yellow band virus, was also immunoprecipitated by anti-PEBV-B serum, confirming the serological relationship between the two viruses.

Keywords: PEBV, translation in vitro, RNA

Present address: Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N OWO.

Received 22 April 1986; accepted 23 June 1986.