J Gen Virol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 67 (1986), 2153-2161; DOI 10.1099/0022-1317-67-10-2153
© 1986 Society for General Microbiology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chui, L. W-L.
Right arrow Articles by Norrby, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chui, L. W-L.
Right arrow Articles by Norrby, E.
Agricola
Right arrow Articles by Chui, L. W-L.
Right arrow Articles by Norrby, E.

Nuclear Accumulation of Measles Virus Nucleoprotein Associated with a Temperature-sensitive Mutant

Linda W-L. Chui, Raija Vainionpää, R. Marusyk, A. Salmi and E. Norrby1

Viral Pathogenesis Research Unit, Department of Medical Microbiology and Infectious Diseases, Faculty of Medicine, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
and1 Department of Virology, Faculty of Medicine, Karolinska Institute, Stockholm, Sweden

A measles virus (MV) Lec strain conditional-lethal (temperature-sensitive) mutant, designated MV ts38, has been isolated from 5-fluorouracil-mutagenized stock. The mutant has been characterized with regard to growth characteristics at 32 °C (permissive temperature) and 39 °C (non-permissive temperature). Virus-specific RNA transcription and/or translation appeared to be blocked at the non-permissive temperature as no virus-specific products could be detected by biochemical or immunological procedures. Following initiation of viral replication at 32 °C, with subsequent shift-up to 39 °C, presynthesized nucleoprotein (NP) was transported to, and accumulated in the cell nucleus whereas other viral proteins could not be detected there. A corresponding accumulation of NP in the nucleus is most often seen in association with MV neurotropic subacute sclerosing panencephalitis isolates in vivo and in vitro.

Keywords: measles virus, nucleoprotein, nucleus, mutant

Received 24 March 1986; accepted 6 June 1986.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1986 by the Society for General Microbiology.