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J Gen Virol 67 (1986), 2215-2222; DOI 10.1099/0022-1317-67-10-2215
© 1986 Society for General Microbiology
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Properties of Natural and Hybrid Murine Alpha Interferons

Margreet Van Heuvel, I. Jaap Bosveld, Arno T. A. Mooren, Jan Trapman and Ellen C. Zwarthoff

Department of Pathology, Erasmus University, P.O. Box 1738, 3000 DR Rotterdam, The Netherlands

Four natural murine interferon-{alpha} genes (MuIFN-{alpha}1, -{alpha}2, -{alpha}4 and -{alpha}6) and four hybrid genes ({alpha}1{alpha}4, {alpha}2{alpha}4, {alpha}4{alpha}1 and {alpha}4{alpha}2) were transiently expressed in monkey COS cells under the transcriptional control of the simian virus 40 early promoter. The proteins were labelled with [35S]methionine during a 16 h incubation and proteins secreted by the cells during this period were separated by polyacrylamide gel electrophoresis and subsequently visualized by fluorography. Under the conditions used, the IFNs represented 5 to 10% of the total amount of secreted proteins. All genes were found to encode biologically active IFN subspecies, including {alpha}4 which has a deletion of five amino acids. When the specific activities of the proteins were compared, it appeared that the specific antiviral activity of {alpha}4 on mouse cells was three- to sixfold higher than the activities of the other natural IFN subspecies. The specific activities of the hybrid proteins were similar to those of the natural proteins, except for the {alpha}2{alpha}4 hybrid which had a higher specific activity than the original proteins. The ability of the natural and hybrid subspecies to protect hamster cells against viral infection was determined using MuIFN-{alpha}1 as a standard. Large differences in activity were found, with {alpha}6 as the most and {alpha}4 as the least active subspecies.

Keywords: interferon (MuIFN-{alpha}) subspecies, hybrid genes, heterologous expression

Received 21 January 1986; accepted 25 June 1986.


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