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Figwort Mosaic Virus DNA Replicates in Cultured Datura stramonium Cells

¹ Dipartimento di Biologia Cellulare, Università degli studi di Camerino, via F. Camerini 2, 62032 Camerino, Italy
and² Department of Virus Research, John Innes Institute, Colney Lane, Norwich NR4 7UH, U.K.

Cellular forms of the DNA of the caulimovirus figwort mosaic virus (FMV), isolated from Datura stramonium leaves and callus cells derived from FMV-infected leaf tissue, have been studied. One- and two-dimensional gel electrophoresis and blot hybridization experiments showed the presence of various truncated forms of FMV DNA reminiscent of the putative replication intermediates of cauliflower mosaic virus (CaMV) thought to be generated by a mechanism involving reverse transcription. FMV DNA was found to be qualitatively and quantitatively similar in leaf and callus tissue, suggesting that, in contrast to CaMV, FMV can multiply efficiently in actively proliferating cells cultured in vitro.

Keywords: caulimovirus, figwort mosaic virus DNA, plant cell culture, supercoiled DNA

Received 17 March 1986; accepted 24 June 1986.