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A Non-structural Protein of Alfalfa Mosaic Virus in the Walls of Infected Tobacco Cells

Laboratoire de Virologie, Institut de Biologie Moléculaire et Cellulaire du Centre National de la Recherche Scientifique, 15 rue Descartes, F-67084 Strasbourg-Cedex, France

The 32000 mol. wt. (32K) non-structural protein of alfalfa mosaic virus, P3, has previously been detected in a crude membrane fraction of infected tobacco leaves, where it accumulated transiently at the beginning of the infection period. We show here, by immunoblotting with an antiserum to a synthetic peptide corresponding to the C terminus of the protein, that the majority of P3 is in the cell wall fraction where it remains throughout infection, both at 25 °C and at 10 °C. The cell wall-associated, P3-related material is heterogeneous and contains polypeptide species of slightly lower electrophoretic mobility than the major in vitro translation product of RNA 3, which suggests that P3 may be post-translationally modified.

Keywords: AIMV, non-structural protein, cell wall

Received 1 April 1986; accepted 9 June 1986.