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J Gen Virol 67 (1986), 2241-2246; DOI 10.1099/0022-1317-67-10-2241
© 1986 Society for General Microbiology
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The 368-Nucleotide Satellite of Cucumber Mosaic Virus Strain Y from Japan Does Not Cause Lethal Necrosis in Tomato

J. M. Kaper, A. S. Duriat{dagger} and M. E. Tousignant

Microbiology and Plant Pathology Laboratory, Plant Protection Institute, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705, U.S.A.

Cucumber mosaic virus (CMV) strain Y from Japan was propagated in Xanthi tobacco. Virus isolated from four successive passages was analysed for its constituent RNAs by polyacrylamide gel electrophoresis (PAGE) under semi-denaturing and fully denaturing conditions. This revealed the initial presence of the 368-nucleotide satellite RNA Y-CARNA 5 (for Y-CMV-associated RNA 5) and the subsequent emergence of a small RNA in the 335-nucleotide size range, which we termed Yn-CARNA 5. The appearance of the smaller Yn-CARNA 5 correlated positively with the increasing tomato necrosis-inducing properties of tissue extracts from the later tobacco passages. After sucrose gradient ultracentrifugation of total CMV-Y RNA, the fraction containing Y-CARNA 5 and Yn-CARNA 5 was subjected to PAGE under semi-denaturing conditions, which provided highly purified gel-eluted preparations of the two CARNA 5 species. When tested for their tomato necrosis-inducing capability in the presence of helper virus genomic RNAs, Y-CARNA 5 was shown to be incapable of eliciting lethal necrosis in Rutgers tomato whereas Yn-CARNA 5 was highly necrogenic.

Keywords: CMV, tomato necrosis, satellite RNA

{dagger} Present address: Lembang Horticultural Research Institute, Jln. Tangkubanperahu 517, Lembang, P.O. Box 587, Bandung, Indonesia.

Received 17 February 1986; accepted 17 June 1986.




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Copyright © 1986 by the Society for General Microbiology.