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Dissociation of the Haemagglutination Inhibition and the Infectivity Neutralization in the Reactions of Influenza A/USSR/90/77 (H1N1) Virus Variants with Monoclonal Antibodies

The D. I. Ivanovsky Institute of Virology of the Academy of Medical Sciences, Gamaleya Street 16, 123098 Moscow, U.S.S.R.

Variants of influenza A/USSR/90/77 (H1N1) virus selected with monoclonal antibody HC 142 (Res 142-1 and Res 142-2) were resistant to this antibody in a virus-neutralization (VN) test, but were inhibited in a haemagglutination-inhibition (HI) test. A variant selected with HC 22 monoclonal antibody (Res 22) was resistant to HC 22 in both VN and HI tests but, like the variants selected with HC 142, was resistant to HC 142 only in VN tests. A mouse-adapted variant of A/USSR/90/77, shown previously to be resistant to HC 22, reacted with HC 142 in a manner similar to that of Res 142-1, Res 142-2 and Res 22. Another monoclonal antibody, HC 125, behaved similarly to HC 142. The addition of anti-immunoglobulin serum restored the ability of HC 142 and HC 125 (already bound to the virus) to neutralize the infectivity of the resistant variants. Polyacrylamide gel electrophoresis revealed differences in the mobility of haemagglutinin among the variants.

Keywords: influenza virus HA, monoclonal antibodies, receptor specificity

Received 17 February 1986; accepted 6 June 1986.

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