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The Stability of Cowpea Mosaic Virus VPg in Reticulocyte Lysates

A. de Varennes

Department of Virus Research, John Innes Institute, Colney Lane, Norwich NR4 7UH, U.K.

The ability of the genome-linked protein (VPg) of cowpea mosaic virus (CPMV) to survive incubation in rabbit reticulocyte lysates was investigated. In contrast to the results obtained with picornavirus RNAs, there was no evidence for the specific removal (‘unlinking’) of the VPg from CPMV RNA during incubation. While linked to RNA, CPMV VPg was protected from proteolytic degradation; if the RNA was first digested with nuclease P1, rapid degradation of the VPg occurred. However if as few as 17 nucleotides were left attached to the VPg, stability was retained.

Keywords: cowpea mosaic virus, reticulocyte lysate, genome-linked protein

Present address: Seccao de Quimica, Instituto Superior de Agronomia, Tapada da Ajuda, 1300 Lisboa, Portugal.

Received 29 May 1986; accepted 1 August 1986.