HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Geryk, J. Articles by Svoboda, J. Search for Related Content PubMed PubMed Citation Articles by Geryk, J. Articles by Svoboda, J. Agricola Articles by Geryk, J. Articles by Svoboda, J.

Characterization of Transforming Viruses Rescued from a Hamster Tumour Cell Line Harbouring the v-src Gene Flanked by Long Terminal Repeats

¹ Institute of Molecular Genetics, Czechoslovak Academy of Sciences, 16637 Prague, Czechoslovakia
and² Department of Microbiology, School of Medicine, University of Missouri, Columbia, Missouri 65212, U.S.A.

The organization of proviruses derived from infecting transforming viruses rescued from hamster tumour cells was studied. Southern blot analysis indicated that the provirus from the F6 cell line was organized as long terminal repeat (LTR)-src-LTR, and S1 mapping experiments suggested that it was probably derived by reverse transcription of src mRNA followed by integration. In the E6 cell line, the provirus unit was arranged as LTR- gag-src-LTR, indicating a recombination event between the rescued transforming virus and the helper virus. These results suggest that transforming defective viruses containing only the src gene can be rescued from non-permissive mammalian cells.

Keywords: proviruses, defective, hamster tumour cells

Received 25 September 1985; accepted 7 July 1986.