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The Genome-linked Proteins of Aphthoviruses: Specific Immunoprecipitation of the Three Species Detected on Virus RNA and Identification of Possible Precursors

¹ Wellcome Biotechnology Ltd, Ash Road, Pirbright GU24 0NQ, U.K.
and² Scripps Clinic and Research Institute, La Jolla, California 92037, U.S.A.

Synthetic peptides have been made corresponding to the C-terminal portion of each of the three presumptive genome-linked proteins (VPgs) of foot-and-mouth disease virus type A₁₀. Antisera against each of these peptides efficiently precipitated only the homologous VPg, and the reactions were inhibited by prior absorption with homologous, but not heterologous synthetic peptide. The peptide antisera precipitated a number of proteins from infected cell extracts with mol. wt. of 100, 84, 56, 36, 27, 25 and 20, all x 10³; all these reactions were inhibited by absorption with homologous peptide, indicating that they were probable precursors of VPg. The relationship between these proteins is at present unclear.

Keywords: FMDV, genome-linked proteins, synthetic peptides

Received 5 March 1986; accepted 7 July 1986.