HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Fraser, R. S. S. Articles by Webb, M. J. W. Search for Related Content PubMed Articles by Fraser, R. S. S. Articles by Webb, M. J. W. Agricola Articles by Fraser, R. S. S. Articles by Webb, M. J. W.

Effects of Anti-microtubule Agents on the Assembly of Tobacco Mosaic Virus Coat Protein

National Vegetable Research Station, Wellesbourne, Warwick CV35 9EF, U.K.

Assembly of the coat protein subunits of tobacco mosaic virus (TMV) into 20S aggregates (‘disks’) and long helices was monitored by light scattering and electron microscopy. The anti-microtubule agent methyl benzimidazol-2-yl carbamate, which inhibits TMV multiplication in vivo, did not affect assembly of coat protein in vitro. In contrast, the anti-microtubule agents colchicine and vinblastine inhibited disk formation, but stimulated rod elongation from coat protein subunits in vitro. Both agents also disrupted preformed disks. Vinblastine inhibited virus multiplication in leaf tissue, but colchicine did not.

Keywords: TMV, coat protein, tubulin, colchicine, vinblastine, MBC

Received 10 April 1986; accepted 7 August 1986.