| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
MRC Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, U.K.
Expression of the early and late genes of herpes simplex virus type 1 (HSV-1) during infection of tissue culture cells requires the prior expression of the immediate early (IE) genes. The requirement for the product of IE gene 3, Vmw175, for the activation of early promoters has been revealed by studies with temperature-sensitive virus mutants. Recent experiments using transfection assays have shown that both Vmw175 and the product of IE gene 1, Vmw110, are involved in the transactivation of a variety of HSV-1 early promoters. This paper describes experiments which compared the activation of two early promoters [those of the glycoprotein gD and thymidine kinase (tk) genes] with that of a member of a later class of genes (the major capsid protein, VP5). Plasmids containing these promoters linked to the chloramphenicol acetyltransferase (CAT) gene were transfected into HeLa cells with plasmids containing one or more HSV-1 IE genes. Promoter activity was estimated by measurement of CAT activity in extracts of transfected cells. The gD and tk promoters were activated by both Vmw175 and Vmw110, and the combination of these two IE gene products resulted in very high levels of activation. Addition of further IE gene products did not result in any significant increase in the activation seen with the combination of Vmw175 and Vmw110. In contrast, the activation of the VP5 promoter brought about by the combination of Vmw175 and Vmw110 was relatively slight, but was increased further when plasmids containing IE gene 2, encoding Vmw63, were included in the transfection. These data suggest that Vmw63, like Vmw175 and Vmw110, is also involved in the activation of transcription from HSV-1 promoters. The effect of Vmw63 may be limited to the activation of a subset of HSV-1 genes.
Keywords: HSV-1, IE genes, transactivation
Received 6 May 1986;
accepted 9 July 1986.
This article has been cited by other articles:
|
|
 |
|
  R. M. Bringhurst and P. A. Schaffer Cellular Stress Rather than Stage of the Cell Cycle Enhances the Replication and Plating Efficiencies of Herpes Simplex Virus Type 1 ICP0- Viruses J. Virol., May 1, 2006; 80(9): 4528 - 4537. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. S. Miller, R. J. Danaher, and R. J. Jacob ICP0 Is Not Required for Efficient Stress-Induced Reactivation of Herpes Simplex Virus Type 1 from Cultured Quiescently Infected Neuronal Cells. J. Virol., April 1, 2006; 80(7): 3360 - 3368. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Stallings, G. J. Duigou, A. A. Gershon, M. D. Gershon, and S. J. Silverstein The Cellular Localization Pattern of Varicella-Zoster Virus ORF29p Is Influenced by Proteasome-Mediated Degradation J. Virol., February 1, 2006; 80(3): 1497 - 1512. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Schwartz, E. E. Brittle, A. E. Reynolds, L. W. Enquist, and S. J. Silverstein UL54-Null Pseudorabies Virus Is Attenuated in Mice but Productively Infects Cells in Culture J. Virol., January 15, 2006; 80(2): 769 - 784. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. S. Ellison, R. A. Maranchuk, K. L. Mottet, and J. R. Smiley Control of VP16 Translation by the Herpes Simplex Virus Type 1 Immediate-Early Protein ICP27 J. Virol., April 1, 2005; 79(7): 4120 - 4131. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Augustinova, D. Hoeller, and F. Yao The Dominant-Negative Herpes Simplex Virus Type 1 (HSV-1) Recombinant CJ83193 Can Serve as an Effective Vaccine against Wild-Type HSV-1 Infection in Mice J. Virol., June 1, 2004; 78(11): 5756 - 5765. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Hagglund and B. Roizman Role of ICP0 in the Strategy of Conquest of the Host Cell by Herpes Simplex Virus 1 J. Virol., March 1, 2004; 78(5): 2169 - 2178. [Full Text] [PDF]
|
|
|
|
|
|
K. D. Perkins, J. Gregonis, S. Borge, and S. A. Rice Transactivation of a Viral Target Gene by Herpes Simplex Virus ICP27 Is Posttranscriptional and Does Not Require the Endogenous Promoter or Polyadenylation Site J. Virol., September 15, 2003; 77(18): 9872 - 9884. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Loiacono, R. Myers, and W. J. Mitchell Neurons Differentially Activate the Herpes Simplex Virus Type 1 Immediate-Early Gene ICP0 and ICP27 Promoters in Transgenic Mice J. Virol., March 1, 2002; 76(5): 2449 - 2459. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-L. Hsu and R. D. Everett Human Neuron-Committed Teratocarcinoma NT2 Cell Line Has Abnormal ND10 Structures and Is Poorly Infected by Herpes Simplex Virus Type 1 J. Virol., April 15, 2001; 75(8): 3819 - 3831. [Abstract] [Full Text]
|
|
|
|
|
|
K. S. Ellison, S. A. Rice, R. Verity, and J. R. Smiley Processing of alpha -Globin and ICP0 mRNA in Cells Infected with Herpes Simplex Virus Type 1 ICP27 Mutants J. Virol., August 15, 2000; 74(16): 7307 - 7319. [Abstract] [Full Text]
|
|
|
|
|
|
P. Cheung, K. S. Ellison, R. Verity, and J. R. Smiley Herpes Simplex Virus ICP27 Induces Cytoplasmic Accumulation of Unspliced Polyadenylated alpha -Globin Pre-mRNA in Infected HeLa Cells J. Virol., March 15, 2000; 74(6): 2913 - 2919. [Abstract] [Full Text]
|
|
|
|
|
|
D. E. Bowles, S. K. Kim, and D. J. O'Callaghan Characterization of the trans-Activation Properties of Equine Herpesvirus 1 EICP0 Protein J. Virol., February 1, 2000; 74(3): 1200 - 1208. [Abstract] [Full Text]
|
|
|
|
|
|
L. E. Hann, W. J. Cook, S. L. Uprichard, D. M. Knipe, and D. M. Coen The Role of Herpes Simplex Virus ICP27 in the Regulation of UL24 Gene Expression by Differential Polyadenylation J. Virol., October 1, 1998; 72(10): 7709 - 7714. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. K. Lium, C. A. Panagiotidis, X. Wen, and S. J. Silverstein The NH2 Terminus of the Herpes Simplex Virus Type 1 Regulatory Protein ICP0 Contains a Promoter-Specific Transcription Activation Domain J. Virol., October 1, 1998; 72(10): 7785 - 7795. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Jordan, J. Pepe, and P. A. Schaffer Characterization of a Nerve Growth Factor-Inducible Cellular Activity That Enhances Herpes Simplex Virus Type 1 Gene Expression and Replication of an ICP0 Null Mutant in Cells of Neural Lineage J. Virol., July 1, 1998; 72(7): 5373 - 5382. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D O'Rourke, G Elliott, M Papworth, R Everett, and P O'Hare Examination of determinants for intranuclear localization and transactivation within the RING finger of herpes simplex virus type 1 IE110k protein J. Gen. Virol., March 1, 1998; 79(3): 537 - 548. [Abstract]
|
|
|
|
 |
|
 R M Sandri-Goldin and G E Mendoza A herpesvirus regulatory protein appears to act post-transcriptionally by affecting mRNA processing. Genes & Dev., May 1, 1992; 6(5): 848 - 863. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
