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J Gen Virol 67 (1986), 2555-2559; DOI 10.1099/0022-1317-67-11-2555
© 1986 Society for General Microbiology
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Sequence Reiteration Required for the Efficient Growth of BK Virus

Kazuya Hara1, Yoshiaki Oya1, Hisashi Kinoshita1, Fumiaki Taguchi1 and Yoshiaki Yogo2

1 Department of Microbiology, School of Hygienic Sciences, Kitasato University, Sagamihara, Kanagawa 228
and2 Department of Viral Infection, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo 108, Japan

Compared with wild-type BK virus DNA having tandem triplication of a 68 base pair (bp) element in its transcriptional control region, a mutant viral DNA with a single copy of the 68 bp element induced remarkably delayed virus production in human embryonic kidney (HEK) cells. We molecularly cloned the DNA of progeny viruses using plasmid vector pAT153. Nucleotide sequence analysis of representative clones revealed that all of the altered viral DNAs examined duplicated various segments extending over origin-distal portions of the 68 bp element and their flanking regions. After transfection of HEK cells, most of these rearranged viral DNAs induced viral growth slightly slower than, or at the same rate as, the wild-type viral DNA. Comparison of the structures of these rearranged viral DNAs suggests that reiteration of a 13 bp sequence, which is located in an origin-distal portion of the 68 bp element, is required for the efficient replication of BK virus.

Keywords: BKV, viral growth, DNA rearrangement, sequence reiteration

Received 22 April 1986; accepted 7 August 1986.


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Copyright © 1986 by the Society for General Microbiology.