HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Rosztóczy, I. Search for Related Content PubMed PubMed Citation Articles by Rosztóczy, I. Agricola Articles by Rosztóczy, I.

Study of the in vivo Priming Effect of Interferon in Mice

Institute of Microbiology, University Medical School, Dóm tér 10, H-6720 Szeged, Hungary

Intramuscular injection of mice with 2000 IU/g partially purified murine interferon (IFN) alpha/beta 3 h before the induction of IFN by intraperitoneally administered 3 µg/g poly rI:rC enhanced early IFN production. The differences between the serum IFN levels of IFN-pretreated and control animals were about 10-fold during the first 2 to 3 h of in vivo IFN production. In later stages these differences tended to decrease, and from 8 h post-induction they disappeared. IFN exerted its in vivo priming activity equally after intravenous, intraperitoneal or intramuscular injection. A similar enhancement of IFN production was observed when it was induced by poly rI:rC administered either intraperitoneally or intramuscularly. The duration of IFN pretreatment influenced the establishment of the in vivo primed state. Following administration of 2000 IU/g murine IFN alpha/beta intramuscularly, maximal priming developed after 3 h, and no primed IFN response was detected when the inoculation of IFN preceded inducer administration by 12 h or more. The manifestation of in vivo priming was optimal when 1500 to 3000 IU/g pretreating doses of IFN were applied. Reduction of the amount of injected IFN below this level markedly decreased priming, indicating a time- and dose-dependent induction of priming in vivo.

Keywords: interferon (MuIFN-/), priming effect, in vivo

Received 22 May 1986; accepted 8 August 1986.

This article has been cited by other articles:

				D. J. J. Carr, L. A. Veress, S. Noisakran, and I. L. Campbell Astrocyte-Targeted Expression of IFN-{alpha}1 Protects Mice from Acute Ocular Herpes Simplex Virus Type 1 Infection J. Immunol., November 1, 1998; 161(9): 4859 - 4865. [Abstract] [Full Text] [PDF]

				W.-S. Yeow, C. M. Lawson, and M. W. Beilharz Antiviral Activities of Individual Murine IFN-{alpha} Subtypes In Vivo: Intramuscular Injection of IFN Expression Constructs Reduces Cytomegalovirus Replication J. Immunol., March 15, 1998; 160(6): 2932 - 2939. [Abstract] [Full Text] [PDF]