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Supercoiling of the Genomic Double-stranded RNA of Rice Dwarf Virus

¹ Tokyo Metropolitan Institute of Medical Science, 3-18-22 Honkomagome, Bunkyo-ku, Tokyo 113
² Department of Phytopathology, Faculty of Agriculture, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156
and³ Department of Applied Chemistry, Faculty of Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113, Japan

When particles of rice dwarf virus were partially disrupted by treatment with EDTA, genome RNA was extruded. Electron microscope observations of these particles suggested that the genome RNA is packed within the virus particle as a supercoiled structure. Genome RNA, extracted using a newly devised two-step extraction method, appeared to consist of supercoiled segments of RNA complexed with protein.

Keywords: RDV, phytoreovirus, supercoiled genome segments

Received 28 April 1986; accepted 7 August 1986.