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Nucleotide Sequence of a cDNA Clone of RNA Segment 10 of Bluetongue Virus (Serotype 10)

Department of Environmental Health Sciences, School of Public Health, University of Alabama at Birmingham, Birmingham, Alabama 35294, U.S.A.

The complete sequence of the double-stranded RNA segment that codes for a non-structural protein (P8) of bluetongue virus serotype 10 has been determined from a cDNA clone inserted into the plasmid pBR322. The segment 10 RNA of the virus (S10 RNA) is deduced to be 822 base pairs long (0.5 x 10⁶ daltons) and has an open reading frame in one strand capable of coding for a protein with a calculated size of 25572 daltons (229 amino acids) and a net charge of +5.5 at neutral pH.

Keywords: BTV, genome segment 10, nucleotide sequence

Received 16 June 1986; accepted 9 September 1986.

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