J Gen Virol
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J Gen Virol 67 (1986), 353-362; DOI 10.1099/0022-1317-67-2-353
© 1986 Society for General Microbiology
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Demonstration of Serological Relationships among Isolates of Barley Yellow Dwarf Virus by Using Polyclonal and Monoclonal Antibodies

R. Diaco1, R. M. Lister3, J. H. Hill2 and D. P. Durand1

1 Department of Microbiology
and2 Department of Plant Pathology, Seed and Weed Sciences, Iowa State University, Ames, Iowa 50011
and3 Department of Botany and Plant Pathology, Purdue University, West Lafayette, Indiana 47907, U.S.A.

Hybridomas secreting monoclonal antibodies against three isolates of barley yellow dwarf virus (BYDV) were established. Two monoclonal antibody preparations were generated against the MAV isolate, one against RPV and six against P-PAV. None of the monoclonal antibody preparations reacted with healthy host components. Reactions of monoclonal antibodies, or unlabelled polyclonal antisera, in an indirect enzyme-linked immunosorbent assay (ELISA) indicated that all three virus isolates share a common epitope. BYDV particles dissociated when incubated in carbonate-bicarbonate coating buffer at pH 9.6 but could be stabilized by prior dialysis against 2% formaldehyde or 2% glutaraldehyde. In indirect ELISA, unlabelled polyclonal antisera bound to both stabilized and dissociated particles of homologous and heterologous BYDV isolates. However, conjugated polyclonal antisera were incapable of binding to dissociated particles or to stabilized particles of heterologous isolates. Experiments with monoclonal antibodies in a competition ELISA indicated the presence of at least two epitopes on the coat protein of P-PAV.

Keywords: BYDV, monoclonal antibodies, ELISA

Received 23 April 1985; accepted 24 October 1985.




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Copyright © 1986 by the Society for General Microbiology.