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Characterization of Monoclonal Antibodies to a U.K. Isolate of Barley Yellow Dwarf Virus

¹ MAFF Harpenden Laboratory, Hatching Green, Harpenden, Herts. AL5 2BD
and² The Monoclonal Antibody Centre, AFRC Institute of Animal Physiology, Babraham, Cambridge CB2 4AT, U.K.

Five rat monoclonal antibodies (MAbs) specific for U.K. isolate B of barley yellow dwarf virus (BYDV) have been produced and tested. The reactions of the MAbs against a range of isolates of known vector specificity from the U.K., U.S.A. and Sweden were compared and a provisional serological correlation suggested. A survey of 90 U.K. field isolates showed that they were of the same serological groupings as the typed isolates, and that the panel of MAbs could be used to characterize field isolates of BYDV. Two of the MAbs were produced in large quantities in ascitic fluids; antibody partially purified from them and conjugated to alkaline phosphatase was used successfully in direct ELISA.

Keywords: BYDV, monoclonal antibodies, serological groups

Received 28 August 1985; accepted 14 November 1985.