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Studies on the Phenomenon of Turnip Yellow Mosaic Virus RNA Release by Freezing and Thawing

Mehrnaz Katouzian-Safadi

Institut Jacques Monod, CNRS and Université Paris VII, 2 Place Jussieu - Tour 43, 75251 Paris Cedex 05, France

In particles of turnip yellow mosaic virus (TYMV), the interactions between proteins are particularly strong when compared to those between proteins in some other icosahedral viruses. Intact RNA is released from TYMV particles on freezing and this process has been studied by examining several parameters that influence cryodenaturation such as dehydration, pressure, aggregation and the presence of protective agents (glycerol and ammonium sulphate). Pressure of 1.5 x 10⁸ Pa had no effect on virus particles whereas dehydration of a virus suspension had a drastic effect. Aggregate formation resulting from freezing of solutions containing high virus concentrations seems to be a prerequisite for RNA release; cryoprotective agents hindered RNA release.

Keywords: TYMV, RNA release, freezing and thawing

Present address: Centre de Biophysique Moléculaire, CNRS, 1A avenue de la Recherche Scientifique, 45045 Orléans Cedex, France.

Received 5 August 1985; accepted 11 November 1985.