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Division of Virology, Department of Pathology, Tennis Court Road, Cambridge University, Cambridge CB2 2QQ, U.K.
The nucleotide sequence of the coding region of the thymidine kinase gene from each of three mutant strains of herpes simplex virus type 1 and from the parental strain, SC16, has been determined. The mutants were known to express thymidine kinase enzymes with distinct substrate binding properties. Consideration of the lesions in the genes responsible for these altered biochemical properties has led us to postulate a preliminary model for the active centre of the enzyme, involving the cooperation of three distinct regions of the polypeptide.
Keywords: HSV-1, thymidine kinase, sequencing of mutants
Present address: Department of Biochemical Virology, Wellcome Research Laboratories, Langley Court, Beckenham, Kent BR3 3BS, U.K.
Received 20 December 1985;
accepted 17 January 1986.
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