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Restriction Enzyme Analysis of Granulosis Viruses Isolated from Artogeia rapae and Pieris brassicae

Glasshouse Crops Research Institute, Worthing Road, Littlehampton, West Sussex BN17 6LP, U.K.

Thirteen isolates of granulosis virus from Artogeia (= Pieris) rapae and two from Pieris brassicae were compared by restriction enzyme analysis. All the isolates gave very similar fragment profiles with XhoI, SmaI and BglI. but at least 11 of them could be distinguished using EcoRI, BstI and HindIII. Similarities and differences between profiles suggested that the isolates could be placed in three subtypes. This subtyping correlated closely with the geographical origin of the isolates, which came from Europe, North America, Asia and Australasia. All the isolates were highly infectious for A. rapae with median lethal dose values for third instar larvae ranging from 10^2·3 to 10^2·6. Only two of four isolates of one subtype had significant infectivity for third instar P. brassicae; thus, this broader host range did not correlate with grouping by restriction enzyme analysis.

Keywords: baculovirus, restriction profiles, GV, host range

Received 6 August 1985; accepted 31 December 1985.